16048 – Enhancing Mesenchymal Stem Cell Efficacy For Cell Therapy

A transiently physical culture condition in the presence of a proprietary cytokine/small molecule cocktail activates MSCs that have already been expanded to be more anti-inflammatory, anti-tumor and immunomodulatory. This method can be applied to MCB/WCBs to prepare activated cryopreserved MSC product cell banks which retain their activity, post thaw. This method can also be applied post-thaw to cryopreserved MSC cell banks. High potency stem cell therapeutic applications create ample opportunities for a variety of applications in regenerative medicine. Researchers at University Health Network have developed a method which alters critical culture conditions under specific and proprietary sequences thereby enabling anti-inflammatory, anti-tumor, and immunomodulatory properties in MSCs. Enhancement in bone marrow- and umbilical cord tissue-MSCs by this proprietary method has been evaluated in vitro by measuring anti-inflammatory protein tumor necrosis factor inducible gene 6 (TSG-6) expression, inhibition of CD4+ T helper (TH) proliferation and in vivo by subsidence of LPS (in paw edema) and zymosan induced (in peritoneum) inflammation. Oksana Akhova oksana.akhova@uhnresearch.ca 4165818569