University of Vienna Background
Up to now, particle-cell interaction experiments on a cell monolayer are predominantly performed in a multiwell plate (e.g. 96-well plate). In this conventional horizontal experimental setup, sedimentation or flocculation characteristics of the particles investigated can greatly influence the outcome of the particle-cell interaction experiment. A fast sedimentation velocity of particles enhances the contact probability with the cell layer leading to an enhanced cellular binding and a higher apparent cytoadhesive capacity measured in a horizontal cell binding experiment. Floating of particles on the other hand leads to a reduced cellular binding and therefore a lower apparent cytoadhesive capacity.
The newly designed cell culture system allows studying cell-particle interactions and performing cell-binding experiments thus giving insight into particle-cell interactions. Furthermore, the new system can mimic the in vivo prevailing shear stress, like for example in the blood vessels, by the moved setting.
The cell culture system eliminates disturbing factors, such as the influence of particle sedimentation or flotation on the cytoadhesive capacity, as the particle suspension is in dynamic flow.
The design also enables further analysis of the cell monolayer, such as immunohistochemistry and microscopy in situ.
Stage of Development
Technology Readiness Level (TRL): 7. Cell culture system-prototype is used in routine laboratory practice.
No influence of sedimentation and flotation
Dynamic flow of particle suspension
Cell monolayer subjected to immunohistochemistry and microscopy
Can be re-used and sterilized
Target customers are research labs.