Detection of H1N1 Influenza A Using Wire-Guide Droplet Manipulations (UA11-031)

This technology is a reprogrammable wire-guided droplet microfluidics tool that can be fabricated into a handheld device for use in field situations. A droplet hangs at the end of a wire or a blunt end syringe needle and moves over a superhydrophobic surface or within a hydrophobic liquid. The differences in the forces of adhesion to the needle wire and to the superhydrophobic surface/liquid enable the movements of a droplet following the movements of the needle wire. The entire polymerase chain reaction (PCR) assay analysis including serial dilution, centrifuging, DNA extraction, thermocycling, and real-time quantification can be finished in less than 15 minutes. The analysis canbe performed in a single device without using any human labor. This has potential for use in COVID-19 testing applications.Background:There have been many attempts to incorporate the complete process of polymerase chain reaction (PCR) assays into microchannels. All of these technologies face the same drawback: users are cannot easily change the assay protocol or chip architecture.Learn more at https://bit.ly/UA11-031 Anne Spieth annes@tla.arizona.edu 520-626-1577