Fluorescence Assisted Genome Editing (FAGE)

University of Luxembourg Technology Overview
Efficient method to derive genome edited populations by the use of fluorescent markers. The Fluorescence Assisted Genome Editing technique allows deriving correctly edited clones with precision genetic modifications. Produced cells are free from non-edited, random integrations and on-target allele NHEJ-containing populations.

Faster than conventional methods. Screening of positive clones can be performed using FACS analysis instead of PCR
Multiple mutations can be generated in high throughput manner creating single nucleotide variants collections


Genetic variants can be deposited in human induced pluripotent stem cells, enabling the generation of any cell type of interest. E.g. brain, muscle or pancreas
Genetic correction allows patients suffering from genetic disorders to obtain healthy cells for tissue therapy


Enable the derivation of isogenic libraries containing mutations relevant to any disease
Isogenic cell libraries permit high-throughput chemical compound screening

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