Fully Integrated Microfluidic Device for Blood Plasma Separation and Biomarker Detection

USC researchers have developed a fully integrated microfluidic device that is capable of blood plasma separation and immunoassay-based disease diagnostic using fluorescence detection. Using bubble-induced acoustic microstreaming (microvortex), the device was able to demonstrate plasma separation with 31.8% yield and 99.9% plasma purity, comparable to traditional centrifuge. Additionally, fluorescent HIVI P24 antibody contained in the plasma mixed with beads where conjugated with P24 antigen. The bound proteins were then captured by acoustic microstreaming and detected using a fluorescence microscope. Bonnie Wolfe bonniep@usc.edu (213) 821-0938