LED array Microscopy Using Multiplexed Illumination Methods and Software

LED array microscopy can be used to achieve gigapixel images with both wide field of view and high resolution. However, the main limitation of the LED array microscope for a full sequential scan of angles is the large amount of data captured and the length of time to acquire the scan. An image must be collected while scanning through each of the LEDs in the array, leading to hundreds of images in each dataset. This is compounded by that fact that each LED has limited intensity, requiring long exposures for each image. To address these problems, UC Berkeley researchers have developed multiplexed illumination methods and software achieving image quality similar to sequential scanning, but with an order of magnitude reduction in both the acquisition time and the data size (number of images) required. Terri Sale terri.sale@berkeley.edu 510-643-4219

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