Nampt Specific Aptamer by a Screening Method based on Graphene

Korea University Background
Need to eliminate loss of the target pool from the existing target immobilization method:

Conventional aptamer development was to fix targets or to fix DNA random libraries
The biggest problem of such an immobilization method is that immobilized yield can be low and it is costly and time-consuming to analyze the immobilized yield
The possibility of DNA being directly bound to a separation material (magnetic beads, columns, etc.) used for immobilization cannot be entirely excluded, and the possibility of loss of a DNA pool which may occur when the DNA bound to a target immobilized to the separation material is separated again remains as a limit and problem of the immobilization method.

Technology Overview
Manufacture of DNA aptamer using graphene:

The mixture solution which comprises single-stranded nucleic acid pool and a target material, is reacted with graphene to remove a single- stranded nucleic acid which is not bound to the target material.
Target materials include Nampt (Nicotinamide phosphoribyltransferase) protein.
A nucleic acid aptamer having a sequence selected from the group consisting of the base sequences set forth in SEQ ID NOS: 4 to 18

: A result of analyzing specificity of DNA aptamers bound to a Nampt protein to adipokines and HAS.
Benefits
Developing an aptamer by using graphene:

Using graphene to remove a single-stranded nucleic acid which is not bound to the target material from mixture solution which comprises single-stranded nucleic acid pool and a target material
Using sequence selected from the group consisting of the base sequences set forth in SEQID NOS: 4 to 18 ()

Developing an aptamer without a process of immobilizing a target:

Since the target is not immobilized, binding sites are not limited, so an aptamer with high bindings to the target can be developed

Efficiently detect Nampt protein using the aptamer:

It is possible to measure concentrations of Nampt protein in the blood that are more sensitive and consistent than that of an antibody-based analysis used previously

Applications
The technology is applied to diagnosis field for diseases such as biomarkers and bio-sensors.
The global aptamers market size was valued at USD 723.6 million in 2016 and is projected to grow at a CAGR of 28.2% during the forecast period.
North America is considered to be the highest revenue generating market with an approximate revenue share of 51% in 2016.
Opportunity
: Built in laboratory environment

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