One-step Surface Modification to Graft DNA Codes on Paper and Its Application for Pathogen Detection

Glass slides have been widely employed for DNA immobilization in DNA microarray in past decades, whereas they are faced with limitations of complicated fabrication procedures, time-consuming modification, and expensive instruments. In this work, a one-step surface modification method with 3-aminopropyl trimethoxysilane (APTMS) was developed and applied, for the first time, for simple DNA immobilization on a paper-based device via the ionic adsorption. X-ray photoelectron spectroscopy (XPS), Infrared spectra (FT-IR) and fluorescence detections were employed to characterize the surface modification and the subsequent DNA immobilization. Incubation conditions of time and temperature were optimized, and the adsorption mechanism was studied systematically. Furthermore, this aminosilane-derivatized paper-based device was successfully applied for the pathogen detection of Giardia lamblia. Compared with conventional methods using abundant cross-linking reactions, this method is simpler, faster and lower-cost, and will broaden the application of paper-based bioassays for point-of-care diagnosis in resource-poor settings. Many DNA code immobilization steps are complicated. we developed a novel one-step surface modification to graft DNA codes on low-cost material of paper and demonstrated its bio-applications, such as pathogen detection. The method is quite simple. The comparison between glass-based methods shows high detection signal of the paper-based methods. The mechanism was further investigated by different methods. The method has great potential for various biological applications, such as pathogen detection, disease diagnosis, environmental monitoring, food safety monitoring and so on. Chao Zhang czhang3@utep.edu 915-747-6717

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