Paper Based Sensor For Detection Of Electron Donors – IP 1428

NAD(P)H and its oxidized form, NAD(P) + are ubiquitous biomolecules associated with cellular energy metabolismin both eukaryotic and prokaryotic organisms. It has been reported that theNAD(P)+/NAD(P)H couples are essential cofactors for more than300 dehydrogenases. Increased activity of dehydrogenases, such as aldehydedehydrogenases, has been reported in various human cancers and has been foundto interfere with certain chemotherapeutic treatments. Several dehydrogenaseinhibitors have been developed for the treatment of human diseases as well asapplications in alcohol dependence, cocaine addiction, anxiety, and asresensitizing agents for cancers. Traditionally, colorimetric detection of NADHis based on the growth of gold nanoparticles, which requires a large samplevolume, a longer reaction time, as well as sophisticated analytical instrumentsto confirm detection efficacy. In addition, it is hard to achieve a lowdetection limit due to the interference arising from background absorbance. FIU inventors have developed diagnostic devices and methods that require less sample volume and easiermethod of fabrication, while maintaining the sensitivity for detecting analytescritical for biological activities in various applications. These devices includea paper-based sensor that takes advantage of the NADH-inhibited dissolution of goldnanoparticles to significantly reduce the background and thereby lower thedetection limit. The sensor surface-confined testing zone minimizes samplevolume, and capillary force-assisted vertical diffusion improves the efficiencyof mass transportation. As a result, the sensor requires only 4 minutes at roomtemperature to analyze a 25 μL sample with a detection limit of 12.5 μM NADH in20% cell lysate by naked-eye examination. Anne Laure Schmitt Olivier aschmitt@fiu.edu 305-348-5948

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