Peptide Surface-modified Nanoparticle for Controlling Freezing

Korea University Background
There is a need to prevent the formation of ice crystals in the biopsied specimen storage industry that low-temperature environments are required.
Freeze preservatives (antifreeze, DMSO, etc.) are generally used to prevent the formation of ice crystals for preventing damage to a biopsied specimen, but it is difficult to apply to since they are toxic in vivo.
Anti-freezing protein (AFP) is being developed as an alternative of freeze preservatives, but increasing the composition of specific amino acids may cause structural deformation and protein may be deposited when high concentration protein is used in biopsied specimen.
Technology Overview
Manufacturing method of peptide surface-modified nanoparticle for controlling freezing:

Peptide is added to a solution containing nanoparticles, mixed at room temperature, and then supernatant is removed through centrifugation.
Peptide surface-modified nanoparticles are manufactured by adding distilled water to the precipitates to redistribute the nanoparticles.
It is confirmed that in case nanoparticles of this technology are applied, more cells are alive than the existing DMSO is applied ().

Benefits
Combining amino acids or peptides on the surface of nanoparticles:

The existing technologies may cause structural deformation when the composition of specific amino acids is increased, and protein may be deposited when high concentration protein is used in biopsied specimen.
This technology may increase the composition of specific amino acids in a genetic engineered way and does not cause precipitation when using high concentrations.
This technology may interact with ice crystals and slow down the speed that ice is produced when freezing.
In addition, it is possible to absorb visible ray or near infrared ray to adjust the melting velocity of ice crystals from the inside of cells.

Nanoparticles that are simple to synthesize and do not precipitate at high concentrations:

The synthesis is simple and thus may reduce the manufacturing process time of nanoparticles.
The damage of cells may be minimized by slowing the speed that ice is produced during refrigeration ().
Apoptosis may be minimized during freeze/unfreeze processes since it is possible to absorb visible ray or near-infrared ray to adjust the melting velocity of ice crystals from the inside of cells.
The damage of biopsied specimen may be minimized during thawing of low-temperature storage specimen.

Applications

This technology can be used to prevent thawing in rivers or seas, or as an antifreeze to prevent freezing of biopsied specimen or in storage fields of frozen food.
This is analyzed with a focus on in vitro diagnostic kit market for diagnosing diseases by using biopsied specimen.
The global market for in vitro diagnostic kit is expected to reach from $23.77 billion in 2015 to $37.82 billion in 2020 at an annual average growth rate of 9.73%.
Reagent and kit fields have the highest share and are expected to have a high growth rate due to the increased needs for in vitro diagnosis and rapid diagnosis of diseases.

Opportunity
– Built in laboratory environment.

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