Spec-Imaging

Lund University Background
The standard and most widely used method for liquid characterization is absorption spectrophotometry. However, for turbid liquids such as dirty water, blood or unfiltered beer, unwanted so-called multiple scattering effects occur, preventing any measurement or introducing large measurement errors. In fact, commercially available spectrophotometers are not capable of measuring within turbid liquids. To reduce the negative effects samples must be diluted prior to measurement. In addition of being time consuming, this procedure introduces dilution errors. Also, in some cases where live monitoring is required – dilution is simply not possible and no reliable solution currently exists.
Technology Overview
The Modu-Spectro incorporates several steps for accurate measurements of the extinction coefficient

Modulated Illumination & multiple scattering suppression
Observation of the exponential decay and exponential fitting
Deducing the extinction coefficient for each wavelength

In the initial testing, the extinction coefficient of milk solution is extracted as shown in the . The conventional Spectrophotometer reaches the limit of transmission measurements at an extinction coefficient of μe=0.50 mm−1. On the contrary, the Modu-Spectro measurements were found to be very reliable, even for an extinction coefficient more than three times as high, μe=1.80 mm−1.
Benefits

No dilution needed
No reference measurement
Online monitoring possibility
Measuring concentration level where commercially available instruments fail

Applications

Beer analysis (color, concentration and turbidity
Pharmaceutics (kinetics and concentration)
Water quality (turbidity and concentration)
Laboratories (CROs)
Food industry
Fluorescence yield quantification

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