UIC-2007-036 – Calibration of Countercurrent and Partition Chromatographs and Columns

High speed countercurrent chromatography (HSCCC) and centrifugal partition chromatography (CPC) are the major techniques that are used for the separation of a large variety of natural products. Proper selection of a biphasic solvent system for counter-chromatography (CCC) separations is very important because in CCC, the solvent acts as both the column as well as the eluant. But the lack of a clear method for comparing the many possible biphasic solvent systems or solvent system family performance seems to be a major impediment in more widespread use of these techniques. The present UIC invention provides a systematic method of evaluation of solvent system families, for the purpose of calibration of CC & CPC chromatographs and columns, as well as rapid development of new CCC/CPC methods. The performance of a particular solvent system is evaluated and the optimum polarity range of solvent systems within a solvent system family is mapped using a mixture of a standard set of compounds called the GUESSmix [Generally Useful Estimation of Solvent Systems (G.U.E.S.S.)]. Once the optimum polarity range of the GUESSmix compounds in a particular solvent system is determined, it provides a reference point for comparing and developing other solvent system families, as well as determining changes in order of elution between solvent system families. The best solvent system choice for the initial test run of an unknown sample is determined based on the separation of GUESSmix standards, and by consideration of all solvent systems in a single family. The GUESSmix standard can also be used for developing and evaluating CCC solvent system families with desired performance characteristics. The present invention can be used as a standard method to evaluate solvent system families, and offers significant advantages to both manufacturers of CCC instruments and the end users alike. Nelson Grihalde grihalde@otm.uic.edu 312-996-4129

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