Ultra-small and Fluorescent Nanoparticles for Live-cell Optical Super-resolution Microscopy

Cornell University Background

Stochastic optical reconstruction microscopy (STORM) is an optical super-resolution microscopy (SRM) technique useful in understanding nanoscale interactions in biological systems
However, STORM traditionally requires toxic imaging buffers and setups not appropriate for live-cell studies
Unmet need: a non-toxic system enabling STORM for live-cell studies

Technology Overview

Cornell researchers have developed ultrasmall (< 10 nm) fluorescent core-shell aluminosilicate nanoparticles (aC’ dots) which covalently encapsulate organic fluorophores for STORM The method uses a single excitation source in a non-toxic imaging buffer to achieve dye blinking, which typically requires two or more excitation sources Functionalization of aC’ dots with antibodies allows for targeted fixed-cell STORM imaging PoC: the aC’ dots enabled quantitative measures of the size of intracellular vesicles and the number of particles per vesicle in live cells Further Details: Erstling, J. A.; Hinckley, J. A.; Bag, N.; Hersh, J.; Feuer, G. B.; Lee, R.; Malarkey, H. F.; Yu, F.; Ma, K.; Baird, B. A.; Wiesner, U. B. Ultrasmall, Bright, and Photostable Fluorescent Core-Shell Aluminosilicate Nanoparticles for Live-Cell Optical Super-Resolution Microscopy. Adv. Mater. 2021, 33, 2006829. https://doi.org/10.1002/adma.202006829 Benefits Dye blinking achieved with only a single excitation source Brighter and more stable probes than free dyes Allows for functionalization (e.g., with antibodies) enabling targeting Quantitative assessment of cellular structures and processes Applications Nanomedicine Live-cell studies

Related Blog

Smart, interactive desk

Get ready to take your space management game to the next level with the University of Glasgow’s innovative project! By combining the

Mechanical Hamstring™

University of Delaware Technology Overview This device was created to allow athletes who suffer a hamstring strain to return to the field

Join Our Newsletter

                                                   Receive Innovation Updates, New Listing Highlights And More