UMIP-326 – Isothermal Point of Care Method for Detection of Human Papilloma Virus (HPV)

Problem: According to the US Centers for Disease Control, “Human Papilloma Virus (HPV) is the most common sexuallytransmitted infection, affecting nearly 80 million people in the US alone, inmost cases, people are infected in their late teens and early 20s, are infectedwith HPV. There are many different types of HPV, many are harmless, but sometypes can cause health problems including genital warts and cancers, inparticular, cervical cancers.” Current commercial methods of detection of high-riskHPV rely mostly on the polymerase chain reaction (PCR) that use either 14specific primer pairs or a single universal primer pair (e.g. GP5+/GP6+).However, PCR requires transport of the sample to a laboratory and the use of athermal cycler (PCR machine) and electricity and therefore is not suitable fora wide-spread, low-cost point of care test. Moreover, these PCR primers are notsuitable for isothermal amplification since at lower temperature they amplifynon-specifically and therefore give high background.” Technology: Researchers at the University of Miami have designed and developed arapid (<30 minutes) isothermal point of care method for thedetection of all 14 high risk HPV subtypes, which works at a constanttemperature (e.g. between 25 and 37°C) using recombinase polymeraseamplification (RPA), . These products can be detected by paper-based lateralflow assays (LFA) using detection probes that are either specific to each HPVsubtype or detect all 14 HPV subtypes at the same time (universal detectionprobe). The result is a detection method that is quick (point-of-care, whilethe patient waits), reliable and low cost (does not necessitate the need forexpensive machinery). Peter Gutenberg pxg372@miami.edu 305-243-4604

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